Antihyperlipidemic and antioxidant activities of “mimicking honey” extract from Basil (Ocimum americanum L.) stem waste

Azza Indrijati Nompo; Afifah Putri Zahrah; Nasywa Fadhiya Pamuji; Nurul Aulia Putri; Abdi Fathir Alfarizki; Lusi Putri Dwita; Ni Putu Ermi Hikmawanti

doi:10.35814/jifi.v24i2.2023

Authors

Azza Indrijati Nompo Undergraduate Program, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, East Jakarta, 13460, Indonesia
Afifah Putri Zahrah Undergraduate Program, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, East Jakarta, 13460, Indonesia
Nasywa Fadhiya Pamuji Undergraduate Program, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, East Jakarta, 13460, Indonesia
Nurul Aulia Putri Undergraduate Program, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, East Jakarta, 13460, Indonesia
Abdi Fathir Alfarizki Undergraduate Program, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, East Jakarta, 13460, Indonesia
Lusi Putri Dwita Department of Pharmacology, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, East Jakarta, 13460, Indonesia
Ni Putu Ermi Hikmawanti Department of Pharmaceutical Biology, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, East Jakarta, 13460, Indonesia

DOI:

https://doi.org/10.35814/jifi.v24i2.2023

Keywords:

Antihyperlipidemic, antioxidant, green solvents, Ocimum americanum L., phenolic

Abstract

Basil (Ocimum americanum L.) has broad pharmacological activity related to its phenolic content. This
compound is a natural antioxidant, which also plays a role in reducing lipid profiles. Phenolics can be extracted with
a “mimicking honey” (one type of the natural deep eutectic solvents, NADES) as an alternative solvent, that is greener
than common volatile organic solvents, such as ethanol. This study aimed to evaluate the antihyperlipidemic and
antioxidant activities of basil stem waste extract prepared using a mimicking honey solvent formulated from water,
sucrose, glucose, and fructose. Extraction was conducted via ultrasonic-assisted methods. An in vivo test was
performed on male Wistar rats induced with hyperlipidemia using quail egg yolk and propylthiouracil. The animals
were divided into eight groups: normal control (no treatment), positive control (atorvastatin), mimicking honey
solvent (MHS), honey solvent (HS), water solvent (WS), basil stem mimicking honey extract (MHE), basil stem honey
extract (HE), and basil stem water extract (WE). Measured parameters included total cholesterol (TC), triglycerides
(TG), and malondialdehyde (MDA) levels. Results indicated that the MHE group showed significantly different levels
of TC (123.00 ± 12.85 mg/dL; P <0.01), TG (85.25 ± 22.29 mg/dL; P <0.001), and MDA (8.10 ± 0.20 nmol/mL; P <0.0001)
compared to the WS group. These findings suggest that synthetic honey solvent is an effective "green solvent" for
extracting bioactive compounds from basil stem, offering potential antihyperlipidemic and antioxidant benefits.

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