Comparative evaluation of magnetic stirrer and homogeniser on globules characterization and stability of red palm oil submicroemulsion prepared by d-phase emulsification method

Elsa Fitria Apriani; Tommy Julianto Bustami Effendi; Windy Keumala Budianti; Mahdi Jufri

doi:10.35814/jifi.v24i1.2042

Penulis

Elsa Fitria Apriani Laboratory of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia
Tommy Julianto Bustami Effendi Departemen Farmasi, Institut Kesehatan Mitra Bunda, Batam, 29454, Indonesia
Windy Keumala Budianti Department of Dermatology and Venereology, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo National Central General Hospital, Jakarta, 10430, Indonesia
Mahdi Jufri Laboratorium Farmasi dan Teknologi Farmasi, Fakultas Farmasi, Universitas Indonesia, Depok, 16424, Indonesia

DOI:

https://doi.org/10.35814/jifi.v24i1.2042

Kata Kunci:

D-Phase Emulsification, physical stability, red palm oil, stirring method, submicroemulsion

Abstrak

Red palm oil (RPO) contains antioxidants due to its carotenoid and vitamin E content. However, the lipophilicity and instability of these two compounds may reduce RPO's bioavailability and efficacy of RPO. This study aimed to develop RPO submicroemulsions and evaluate the effects of magnetic stirrer (M) and homogeniser (H) on their characteristics and physical stability. A submicroemulsion was developed using the D-phase emulsification method. Formulas with globule sizes less than 200 nm, polydispersity index (PDI) less than 0.4, and zeta potential less than -30 mV were tested for stability at room temperature for 3 months and analysed for Ostwald ripening rates. The homogenizer method produced smaller globule sizes and lower PDI (p<0.05). Ten formulations from both the magnetic stirrer and homogeniser methods met the initial globule requirements. After 3 months, F4H and F9H showed the best physical stability with globule sizes of 181.23±0.96 and 175.23±1.88 nm, PDI of 0.077±0.034 and 0.035±0.023, and zeta potential of -33.80±0.56 and -30.57±0.15 mV (p>0.05). However, F9H had a lower Ostwald ripening rate than F4H, namely 2.54×10⁵ and 6.04×10⁵ nm³/month, respectively. In conclusion, the homogeniser produced more stable RPO submicroemulsions than the magnetic stirrer.

Referensi

[1] B. S. R. Zangana and K. O. J. Al-Bahadly, "Estimating of fatty acids, tocopherols, tocotrienols, total carotenes, study the physiochemical properties and unsaponifiable matters extraction from crude RPO," Baghdad Sci J, vol. 14, no. 2, pp. 254–261, 2017. doi: 10.21123/bsj.14.2.254-261.

[2] M. Nainggolan and A. G. S. Sinaga, "Characteristics of fatty acid composition and minor constituents of red palm olein and palm kernel oil combination," J Adv Pharm Technol Res, vol. 12, no. 1, pp. 22–26, 2021. doi: 10.4103/japtr.JAPTR_91_20.

[3] S. Hidayati, F. Nurainy, E. Suroso, D. Sartika, and S. Hadi, "Effect of heating time on changes in physicochemical properties and fatty acid composition of RPO," Afr J Food Agric Nutr Dev, vol. 24, no. 2, pp. 25628–25644, 2024. doi: 10.18697/ajfand.127.23005.

[4] J. Bufka, L. Vaňková, J. Sýkora, and V. Křížková, "Exploring carotenoids: Metabolism, antioxidants, and impacts on human health," J Funct Foods, vol. 118, p. 106284, 2024. doi: 10.1016/j.jff.2024.106284.

[5] J. Fiedor and K. Burda, "Potential role of carotenoids as antioxidants in human health and disease," Nutrients, vol. 6, pp. 466–488, 2014. doi: 10.3390/nu6020466.

[6] C. C. Chu, S. C. Chew, W. C. Liew, and K. L. Nyam, "Vitamin E: A multi-functional ingredient for health enhancement," J Food Meas Charact, vol. 17, pp. 6144–6156, 2023. doi: 10.1007/s11694-023-02042-z.

[7] T. E. Tallei, B. J. Kepel, H. I. S. Wungouw, F. Nurkolis, A. A. Adam, and Fatimawali, "A comprehensive review on the antioxidant activities and health benefits of microgreens: current insights and future perspectives," International Journal of Food Science and Technology, vol. 59, no. 1, pp. 58–71, Jan. 2024. doi: 10.1111/ijfs.16805.

[8] A. Muršec, B. Poljšak, A. N. Svete, and V. Erjavec, "Antioxidant Strategies for Age-Related Oxidative Damage in Dogs," Veterinary Sciences, vol. 12, no. 10, p. 962, 2025. doi: 10.3390/vetsci12100962.

[9] M. S. Islam and S. Mitra, "Effect of nano graphene oxide (nGO) incorporation on the lipophilicity of hydrophobic drugs," Hybrid Advances, vol. 3, p. 100074, 2023. doi: 10.1016/j.hybadv.2023.100074.

[10] Y. Khan et al., "Role of Decomposition on Drug Stability," in Drug Stability and Chemical Kinetics, Singapore: Springer Nature, pp. 83-94, 2020. doi: 10.1007/978-981-15-6426-0_7.

[11] V. Mundada, M. Patel, and K. Sawant, "Submicron Emulsions and Their Applications in Oral Delivery," Crit Rev Ther Drug Carrier Syst, vol. 33, no. 3, pp. 265-308, 2016. doi: 10.1615/CritRevTherDrugCarrierSyst.2016017218.

[12] M. Almeida, H. Teixeira, and L. Koester, "Preparation of Submicron Emulsions: Theoretical Aspects about the Methods Employed Today," Latin American Journal of Pharmacy, vol. 27, pp. 780-788, 2008.

[13] Y. Chao et al., “The effect of submicron emulsion systems on transdermal delivery of kaempferol,”Chem Pharm Bull (Tokyo), vol. 60, no. 9, pp. 1171-1175, 2012. doi: 10.1248/cpb.c12-00372.

[14] H. Jasmina et al., “Preparatıon of Nanoemulsıons By Hıgh-Energy and Lowenergy Emulsıfıcatıon Methods,” in Badnjevic, A. (eds) CMBEBIH 2017, IFMBE Proceedings, Singapore: Springer, 2017. doi:10.1007/978-981-10-4166-2_48

[15] J. S. Komaiko and D. J. McClements, "Formation of Food-Grade Nanoemulsions Using Low-Energy Preparation Methods: A Review of Available Methods," Compr Rev Food Sci Food Saf, vol. 15, no. 2, pp. 331-352, Mar. 2016. doi: 10.1111/1541-4337.12189.

[16] B. Lin et al., "Preparation and mechanism investigation of surfactin-based nanoemulsion by D-phase emulsification," Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 714, p. 136613, 2025. doi: 10.1016/j.colsurfa.2025.136613.

[17] S. Zhou et al., “Influence of structural parameter variations of high-shear mixers on emulsion particle size,” Chemical Engineering Research and Design, vol. 223, pp. 511-523, 2025. doi: 10.1016/j.cherd.2025.10.020

[18] N. Michalak et al., "Ostwald Ripening in an Oxide-on-Metal System," Advanced Materials Interfaces, vol. 9, no. 17, p. 2200222, 2022. doi: 10.1002/admi.202200222.

[19] M. N. Yukuyama et al., "High internal vegetable oil nanoemulsion: D-phase emulsification as a unique low energy process," Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 554, pp. 296–305, 2018. doi: 10.1016/j.colsurfa.2018.06.023.

[20] Miksusanti, E. F. Apriani, and A. H. B. Bihurinin, "Optimization of Tween 80 and PEG-400 Concentration in Indonesian Virgin Coconut Oil Nanoemulsion as Antibacterial against Staphylococcus aureus," Sains Malaysiana, vol. 52, no. 4, pp. 1259–72, Apr. 2023.

[21] E. F. Apriani, M. Mardiyanto, and A. Hendrawan, "Optimization of Green Synthesis of Silver Nanoparticles From Areca catechu L. Seed Extract With Variations of Silver Nitrate and Extract Concentrations Using Simplex Lattice Design Method," Farmacia, vol. 70, no. 5, pp. 917–24, 2022.

[22] W. Park, J. Park, S. Im, and S. J. Choi, "Influence of the type and concentration of hydrocolloids on Ostwald ripening of emulsions stabilized with small molecular and non-ionic surfactants," Food Chem, vol. 411, p. 135504, 2023.

[23] J. Fukutomi et al., "Study of Emulsification Mechanism in a Pressure Type Homogeniser," TRANSACTIONS OF THE JAPAN SOCIETY OF MECHANICAL ENGINEERS Series B, vol. 79, no. 806, pp. 2030-2040, 2013. doi: 10.1299/kikaib.79.2030.

[24] T. Sumitomo et al., "Study of Internal Flow and Emulsification Effect in a Homogeniser(Fluids Engineering)," Transactions of the Japan Society of Mechanical Engineers Series B, vol. 75, no. 759, pp. 2199-2206, 2009. doi: 10.1299/kikaib.75.759_2199.

[25] E. Sokolov, D. Kalyuzhnaya, and P. Ryapolov, "Behavior of “Non-Magnetic Liquid-Magnetic Fluid” Emulsions in Microchannels Under the Influence of an Inhomogeneous Magnetic Field," IEEE Transactions on Magnetics, vol. 61, no. 9, pp. 1-5, Sept. 2025. Art no. 9200705. doi: 10.1109/TMAG.2025.3544657.

[26] N. T. Nguyen, S. H. Tan, and J. Liu, "Magnetically Mediated Formation of Ferrofluid Emulsion," in ASME 2011 9th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM 2011, vol. 2, 2011. doi: 10.1115/ICNMM2011-58212.

[27] E. M. Uhlig and A. S. Khair, "Double-layer structure and interfacial tension at an ionic surfactant-laden interface," Journal of Colloid and Interface Science, vol. 697, p. 137924, 2025. doi: 10.1016/j.jcis.2025.137924.

[28] A. A. Shahir, A. V. Nguyen, and S. I. Karakashev, "A quantification of immersion of the adsorbed ionic surfactants at liquid|fluid interfaces," Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 509, pp. 279-292, 2016. doi: 10.1016/j.colsurfa.2016.08.082.

[29] Y. Shen and H. Hoffmann, "Formation of Unique Unilamellar Vesicles from Multilamellar Vesicles under High-Pressure Shear Flow," J Phys Chem B, vol. 122, no. 37, pp. 8706-8711, Sep. 2018. doi: 10.1021/acs.jpcb.8b04646.

[30] L. Zhou et al., "Effects of high-speed shear homogenization on the emulsifying and structural properties of myofibrillar protein under low-fat conditions," J Sci Food Agric, vol. 99, no. 14, pp. 6500-6508, Nov. 2019. doi: 10.1002/jsfa.9929.

[31] T. Lorenz and A. Dietzel, "Microfluidic 3D flow-focusing for precipitation and concentration of drug nanoparticles," in MikroSystemTechnik Kongress 2017 "MEMS, Mikroelektronik, Systeme", Proceedings, pp. 238-241, 2017.

[32] S. Chono, "Development of drug delivery systems for targeting to macrophages," Yakugaku Zasshi, vol. 127, no. 9, pp. 1419–1430, 2007. doi: 10.1248/yakushi.127.1419.

[33] Z. Jiang et al., "Small tumour microparticle enhances drug delivery efficiency and therapeutic antitumour efficacy," Cancer Nano, vol. 13, p. 19, 2022. doi: 10.1186/s12645-022-00125-y.

[34] R. Su et al., "Size-dependent penetration of nanoemulsions into epidermis and hair follicles: implications for transdermal delivery and immunization," Oncotarget, vol. 8, no. 24, pp. 38214-38226, Jun. 2017. doi: 10.18632/oncotarget.17130.

[35] S. Daware, V. Raje, A. Patel, and K. Patel, "Investigating Key Molecular Descriptors Affecting Particle Size: A Predictive Exemplary Approach for Self-Emulsifying System," Molecular Pharmaceutics, vol. 20, no. 5, pp. 2556-2567, 2023. doi: 10.1021/acs.molpharmaceut.2c01118.

[36] Q. Zhang et al., "Effect of oil structure on adsorption behavior of emulsifier at the oil-polyol interface and the emulsion features," Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 703, p. 135198, 2024. doi: 10.1016/j.colsurfa.2024.135198.

[37] R. Zhao et al., "Cutoff Ostwald ripening stability of eugenol-in-water emulsion by co-stabilization method and antibacterial activity evaluation," Food Hydrocolloids, vol. 107, p. 105925, 2020. doi: 10.1016/j.foodhyd.2020.105925.