Response of Leuconostoc mesenteroides and Weissella confusa strains on pH of Growth Condition using Antibiotics as Indicator

Authors

  • AMARILA MALIK UNIVERSITAS INDONESIA ,
  • DEWI PUSPITA UNIVERSITAS INDONESIA ,
  • RIZKI AYU AMALIA UNIVERSITAS INDONESIA ,

Keywords:

lactic acid bacteria, growth response, Leuconostoc mesenteroides, Weissella confusa, antibiotic, acidic modified MRS, alkaline modified MRS

Abstract

Lactic acid bacteria is known to contribute to human health as exopolysaccharide producer, bacteriocin producer, live vaccins, starter in fermentation process and as probiotics. This study aimed to observe Leuconostoc mesenteroides and Weisella confusa strains response under manipulated growth condition for carbon source composition and on pH as a model, using various antibiotics as indicator. MRS and modified MRS (50% normal dextrose concentration) were used. Modification of pH representing acidic and alkaline condition were 4.6 and 9.0. Disc diffusion method was employed using six different antibiotics. Result showed that all strains grown in acidic standard MRS tend to be more sensitive to amoxicillin (Am), chloramphenicol (Ch) and polymyxin B (PoB), but more resistant to ciprofloxacin (Ci), while in the acidic modified MRS, the strains respond more sensitive to Am, Ch, Ci and PoB. When grown in alkaline standard MRS, all strains respond more sensitive to Am, Ci and PoB, but more resistant to Ch, while in alkaline modified MRS, they respond more resistant to Am and Ch but more sensitive to Ci and PoB. Most of the strains did not show inhibition against vancomycin and none of strains showed inhibition against sulphamethoxazole-trimethoprim under conditions applied.

References

Downloads

Published

2013-09-30

Issue

Vol. 11 No. 2 (2013): JIFI

Section

Articles

License

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.