Optimization of Hydroxy Octadecadienoic Acid (HODE) Production by Culvularia lunata BioMCC FE-00283 Using Response Surface Methodology

Authors

  • ERWAHYUNI ENDANG PRABANDARI INSTITUT PERTANIAN BOGOR ,
  • TUN TEDJA IRAWADI INSTITUT PERTANIAN BOGOR ,
  • WAHONO SUMARYONO BADAN PENGKAJIAN DAN PENERAPAN TEKNOLOGI ,
  • KHASWAR SYAMSU INSTITUT PERTANIAN BOGOR ,

Keywords:

optimization, RSM, hydroxy octadecadienoic acid, endophytic fungi, Culvularia lunata

Abstract

Hydroxyl octadecadienoic acid (HODE) is an oxidized aliphatic fatty acid from the oxylipins group, which shows bioactivity and can be produced by Curvularia lunata BioMCC FE-00283 by submerged fermentation. Glucose, monosodium glutamate, and corn oil were media components that showed significant effect toward HODE production by C. lunata BioMCC FE-00283. Composition optimization of these three medium components was carried out by Response surface methodology (RSM). A full factorial central composite design (CCD) was used to explain the interaction effect between media components. Maximum HODE production as predicted by the quadratic model have chromatogram area of 4540000, which was verified experimentally to be 1091338 Â± 152489. Eventhough experimental veriiication showed lower result compared to the model prediction, however it was higher compared to the HODE production using basal media with unoptimized composition of carbon, nitrogen, and inducer which only have chromatogram area of 295045 Â± 71200. The verification also showed that optimization increased HODE production by 3.7 fold compared to that produced by the unoptimized media.

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Published

2011-04-30

Issue

Vol. 9 No. 1 (2011): JIFI

Section

Articles

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