Partial Validation of High Performance Liquid Chromatography for Analysis of Isoniazid in Rat Plasma

Authors

  • Novi Yantih UNIVERSITAS PANCASILA ,
  • Siti Hafilah UNIVERSITAS PANCASILA ,
  • Yahdiana Harahap UNIVERSITAS INDONESIA ,
  • WAHONO SUMARYONO UNIVERSITAS PANCASILA ,
  • Rianto Setiabudy UNIVERSITAS INDONESIA ,

DOI:

https://doi.org/10.35814/jifi.v16i1.497

Keywords:

Isoniazid, high performance liquid chromatography, rat plasma

Abstract

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Nicotinic acid derivatives such as isoniazid have the strongest anti-tuberculosis properties. For pharmacokinetics studying of isoniazid (INH), a method is needed to determine the levels of INH in plasma. Objective: The aim of this research is to partial validate of high performance liquid chromatography (HPLC) for analysis of INH in rat plasma. Method: For the preliminary study, rat plasma was used. The HPLC system used is a stationary phase C18 with length 250mm and temperature of 30°C, mobile phase hexane sulphonate acid 20mM pH 2.47–methanol (65:35). The analytical parameters in partial validated were linearity, lower limit of quantification (LLOQ), precision, accuracy, and recovery. Results: The results of linearity test of INH showed r value of 0.9996. LLOQ of this method was 0.1258μg/mL. The resulting accuracy and precision value met FDA requirements with a percent recovery ranging from 96.57–107.99%. Conclusion: The HPLC system was a valid method for analysis of INH in rat plasma.

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Published

2018-04-27

Issue

Vol. 16 No. 1 (2018): JIFI

Section

Articles

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