Determination of Vitamin E Levels in n-Hexane Extract of Palm Fruits (Elaeis guineensis Jacq) and CPO (Crude Palm Oil) using HPLC Method

Authors

  • Mustika Furi Sekolah Tinggi Ilmu Farmasi Riau (STIFAR Riau) ,
  • Rahma Dona Sekolah Tinggi Ilmu Farmasi Riau (STIFAR Riau) ,
  • Vincent Tristan Sekolah Tinggi Ilmu Farmasi Riau (STIFAR Riau) ,

DOI:

https://doi.org/10.35814/jifi.v20i1.915

Keywords:

Vitamin E, palm oil, CPO, HPLC

Abstract

Palm fruit (Elaeis guineensis Jacq) is a natural source of vitamin E (tocopherol). This study has determined vitamin E levels in n-hexane extracts of palm fruit (Elaeis guineensis Jacq) and CPO (Crude Palm Oil) using HPLC (High Performance Liquid Chromatography) method. The subject were palm fruit and CPO obtained from plantations and palm fruit mills PT X located in Kampar district, Riau. Extraction of palm fruit and CPO using ultrasonication and saponification method. Measurement using HPLC obtained optimum conditions using mobile phase acetonitrile: water (86:14), flow 1 mL/minute, UV-Vis detector at wavelength 284 nm and obtained retention time is 3,488 minutes.  Determination of the calibration curve is obtained the regression equation y = 702,872x + 3273,26 and r2 = 0,9985. The result of the determination vitamin E levels in the palm fruit extract and CPO sequentially is (469,4008 ± 2,5879) µg/mg and for CPO is (262,5248 ± 6,3987) µg/mg.

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Published

2022-04-29

Issue

Vol. 20 No. 1 (2022): JIFI

Section

Articles

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