Determination of Flavonoid Content and Anti-Inflammatory Activity Extract and Fraction of Sungkai Leaf (Peronema canescens Jack)

Authors

  • Desi Nadya Aulena Departement of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Pancasila, South Jakarta, Jakarta, 12640, Indonesia ,
  • Dwi Fitri Yani Departement of Chemistry, Faculty of Sains and Technology, Universitas Islam Negeri Raden Fatah Palembang, South of Sumatera , 30257 , Indonesia ,
  • Mariyamah Mariyamah Departement of Chemistry, Faculty of Sains and Technology, Universitas Islam Negeri Raden Fatah Palembang, South of Sumatera , 30257 , Indonesia ,
  • Muhammad Lufika Tondi Departement of Chemistry, Faculty of Sains and Technology, Universitas Islam Negeri Raden Fatah Palembang, South of Sumatera , 30257 , Indonesia ,
  • Muhammad Dandi Departement of Chemistry, Faculty of Sains and Technology, Universitas Islam Negeri Raden Fatah Palembang, South of Sumatera , 30257 , Indonesia ,
  • Hafis Kiki Wahyudin Departement of Chemistry, Faculty of Sains and Technology, Universitas Islam Negeri Raden Fatah Palembang, South of Sumatera , 30257 , Indonesia ,
  • Dany Raihan Faculty of UBC Vantage College, University of British Colombia, Vancouver V6T 1Z4, Canada ,

DOI:

https://doi.org/10.35814/jifi.v21i2.1437

Keywords:

COVID-19, inflammation, infectious disease, protein denaturation, SARS-CoV-2 virus

Abstract

The SARS-CoV-2 virus is the infectious agent that causes COVID-19, a feverish condition brought on by inflammation in the infected patient’s body. Sungkai leaf (Peronema canescens Jack) is one of the Indonesian people who rely on herbal remedies to treat COVID-19. This study aims to determine how much flavonoids are present in ethanol, ethyl acetate, and n-hexane extracts, as well as the anti-inflammatory properties of ethanol extract, ethanol fraction, and n-hexane fraction of Sungkai leaves. The procedure was performed in-vitro with a UV-Visible spectrophotometer by observing the absorption response to inhibition of denaturation of inflammatory protein. The inhibition value was then computed via linear regression, and the IC50 and IC70 values were ascertained afterward. The ethanol fraction, ethanol extract, and n-hexane fraction in this investigation had the best inhibition values (%) at a concentration of 15 ppm, corresponding to 74.27%, 54.48%, and 18.52%. The sungkai leaves ethanol fraction > n-hexane fraction > ethanol extract had the best IC50 and IC70 values. Comparatively, the ethanol extract contained the largest amounts of flavonoids, 38.782 μg/mL.

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Published

2023-10-16

Issue

Vol. 21 No. 2 (2023): JIFI

Section

Articles

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