Phagocytic Macrophage Activity of Fractions from Methanolic Leaf Extract of Red Betel (Piper crocatum Ruiz & Pav.) In Vitro

Authors

  • YUSTINA SRI HARTINI UNIVERSITAS SANATA DHARMA ,
  • SUBAGUS WAHYUONO UNIVERSITAS GADJAH MADA ,
  • SITARINA WIDYARINI UNIVERSITAS GADJAH MADA ,
  • AGUSTINUS YUSWANTO UNIVERSITAS GADJAH MADA ,

Keywords:

red betel, Piper crocatum Ruiz & Pav., fractions, methanolic extract, phagocytic macrophage, immunomodulator

Abstract

Plants can be sources of immunomodulatory agents. Piper crocatum Ruiz & Pav. (red betel) leaves have been used to heal many diseases, especially to increase endurance. The aim of this research was to investigate the imunomodulatory effect of methanolic leaf extracts of Piper crocatum Ruiz & Pav. and its fractions by in vitro phagocytic macrophage activity test. The result showed that methanolic extract of Piper crocatum Ruiz & Pav. 75μg/mL could stimulate phagocytic macrophage activity equal to 100μg/mL product-X® that contained Echinacea extract. Separation of the extract by vacuum liquid chromatography using gradient solvent n-hexane - ethyl acetate yielded 5 fractions. The second fraction showed the highest activity. It contained alkaloid and terpenoid, while the extract also contained flavonoid and essential oil. Alkaloid and/or terpenoid could be the compound in the leaves of Piper crocatum Ruiz & Pav. that responsible increasing phagocytic macrophage activity.

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Published

2013-09-30

Issue

Vol. 11 No. 2 (2013): JIFI

Section

Articles

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