SPACE-Peptide succesfully improves dermis penetration of amniotic membrane stem cell metabolite product as antiaging

Diah indah kumala Sari; Safira Yulita Fazadini

doi:10.35814/jifi.v24i2.2048

Authors

Diah indah kumala Sari Department Pharmacy, Faculty of Health Sciences, Universitas Muhammadiyah Lamongan, Lamongan, 62211, Indonesia
Safira Yulita Fazadini Department Pharmacy, Faculty of Health Sciences, Universitas Muhammadiyah Lamongan, Lamongan, 62211, Indonesia

DOI:

https://doi.org/10.35814/jifi.v24i2.2048

Keywords:

AMSCMP, irritation, penetration, SPACE-Peptide

Abstract

Amniotic Membrane Stem Cell Metabolite Product (AMSCMP) is a totipotent stem cell derived metabolite with contains protein size of 75.33 kDa so it is difficult to penetrate the dermis. Skin Penetrating and Cell Entering (SPACE) proven to facilitate protein molecule penetration. The advantage of SPACE its reversible and non irritating. This study was to assess the parameters of successful penetration and the level of skin irritation after AMSCMP was added with SPACE Peptide. The method used is in vivo research using test animals, then penetration analysis is seen from measuring the depth of staining, while the penetration test looks at the changes that occur in the skin after the sample is applied in the form of edema, PMN and degenerative. Penetration tests showed differences in color fluorescence before and after the addition of SPACE peptide. This indicates that the parts of the mouse skin that exhibited color fluorescence had better penetration (F2, F3, F4) compared to those that did not fluoresce (F1). The F1 measurement results did not have a penetration value, while F2, F3, and F4 had values of more than 1200 micrometers. The irritation test showed that F1 and F2 had no irritation index (zero) with edema, polymorphonuclear, and degenerative parameters, while F3 and F4 had a value of 1.3 from the average results with a very mild irritation category. The study results demonstrated that the addition of SPACE significantly increased the penetration of the formulation. Based on statistical analysis using SPSS, the penetration parameter differed significantly among the formulas (p<0.05), indicating that the addition of SPACE had a significant effect on enhancing the penetration of the active ingredient. In contrast, the irritation test results showed that the addition of SPACE caused irritation in the very mild category. Nevertheless, statistical analysis revealed no significant difference in irritation scores among the formulas (p > 0.05), suggesting that the addition of SPACE did not significantly affect the degree of irritation caused.

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