Effect of extract and Salung Leaf Fraction (Psychotria viridiflora Reinv. Ex. Blume) As Sitotoksit, Antiproliferation and Applose of Apoptosis on Cancer Cell of Serviks HeLa

Abstract

Cervical cancer ranks second of cancer that affects women in the world and the first order for women in developing countries. Plant Salung (Psychotria viridiflora Reinw. Ex. Blume) have been used for generations to treat various diseases. The purpose of the study to determine the effects of leaf extracts and fractions salung as the anti-proliferation and inducer of apoptosis in HeLa cervical cancer cells. Concentration of extracts and fractions of 640; 320; 160; 80; 40 µg/mL, while cisplatin using a concentration of 200; 100; 50; 25 µg/mL. The results showed IC₅₀ extract value 380.7 µg/mL. n-hexane fraction amounted to 229.3 µg/mL. ethyl acetate fraction of 116.8 µg/mL, and amounted to 562.8 µg/mL fraksi methanol water, so that the ethyl acetate fraction had enough categories active cytotoxic activity seen from the IC₅₀. To further test the researchers only tested the active fraction is the fraction of ethyl acetate. Results ethyl acetate fraction doubling time is 58, which means replicating or proliferating cells on hour to 58 while the cells replicate cisplatin on the hour to 64.5 and the control cell on the hour to 41. Results of flowcytometry showed ethyl acetate fraction induce apoptosis by 72.82% while cisplatin 87.96%. It can be concluded that the extracts and fractions of leaves salung have a cytotoxic effect, acts as an anti-proliferation and inducer of apoptosis in HeLa cervical cancer cell.