Identification of α-glucosidase Enzyme Inhibitor Compound from Ethanol 96% Extract of Yakon Leaves (Smallanthus sonchifolius [Poepp.& Endl.] H. Robinson)

Authors

  • Zuhelmi Aziz Universitas Pancasila ,
  • Farhana Hanida Al Qisthi Universitas Pancasila ,
  • Ratna Djamil Universitas Pancasila ,
  • Nancy Dewi Yuliana Faculty of Argicultur Technology, Institut Pertanian Bogor ,
  • Partomuan Simanjuntak Pusat Penelitian Bioteknologi-LIPI ,
  • Syamsudin Abdillah ,

DOI:

https://doi.org/10.35814/jifi.v17i1.652

Keywords:

Yakon, smallanthussonchi folius [Poepp. &Endl.] H. Robinson, antidiabetic, α-glucosidase, nystose

Abstract

The leaves of yacon (Smallanthussonchi folius [Poepp. &Endl.] H. Robinson) or in Indonesia known as “Daun insulin†(Insulin leaf), scientifically has various medicinal properties and one of them as antidiabetic. The aim of this research is to know α-glucosidase enzyme inhibitor compound in the ethanol 96% extract of yacon leaves. Fractionation of ethanol extract subjected to column chromatography (SiO2; dichloromethane-methanol-water = 5 : 5 : 1) gave 5 fractions (Fr -1 ~Fr – 5). The results of the ï¡-glucosidase enzyme inhibition activity test in vitro against the five fractions obtained the 3rd fraction as the most active of 87.84 %. Purification of the 3rd fraction with preparative thin layer chromatography (SiO2; n-BuOH-ethanol-water = 4 : 1 : 2.2) gave pure isolate as Fr 3-1. Identification of pure isolates Fr 3-1 was carried out by interpreted spectra of Ultraviolet, Fourier Transform-Infra Red, Mass Spectra (from LC-MS) and compared data on the chemical shift of the Nuclear Magnetic Resonance (proton and carbon) and determined as Nystose (GF3) compound. The results of the ï¡-glucosidase enzyme inhibition activity showed that      Fr 3-1 has IC50 of 21.36 ppm

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Published

2019-04-24

Issue

Vol. 17 No. 1 (2019): JIFI

Section

Articles

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