Antidiarrheal Activity Test of White Pomegranate (Punica granatum L.) Infusion on Swiss Webster Male with Oleum Ricini Method

Abstract

Diarrhea is a condition in which a person defecates with a soft or liquid consistency, which can even
be water. Diarrhea can be treated with drugs derived from plants, one of which is white pomegranate skin. The
skin of white pomegranate (Punica granatum L.) is thought to have anti-diarrheal activity because it has fl avonoids
and tannins. This study aims to determine the activity and optimal dose of pomegranate skin infusion.
Mice were grouped into 5 groups: negative control (Na CMC 0.5%), positive control (Loperamid HCl), and
pomegranate peel infusion test group with a dose of 8 mg / 20 g mice BW, 16 mg / 20 g weight mice and 32 mg.
/ 20 gr BB mouse. Mice induced diarrhea with Oleum ricini, 30 minutes later the mice were given a test preparation.
The onset of diarrhea, stool consistency, diarrhea frequency, duration of diarrhea and stool weight were
measured every 30 minutes for 6 hours. The results showed that the fi rst dose (8 mg / 20 gr BW of mice) was
the optimal dose for the initial parameters of diarrhea and fecal weight, which means that there was a signifi cant
diff erence with negative control and positive control (<0.05). The parameters of stool consistency, frequency of
diarrhea and duration of occurrence of diarrhea showed no signifi cant diff erence between groups of 8 mg / 20 g
mice BW, 16 mg / 20 g mice BW and 32 mg / 20 g mice BW, negative control and positive control (p> 0, 05).