Uji Aktivitas Penghambatan Enzim α-Glukosidase serta Uji Mutu Ekstrak Etanol Batang Brotowali (Tinospora crispa (L.) Miers.)

Penulis

  • YESI DESMIATY UNIVERSITAS PANCASILA ,
  • RISMA MARISI TAMBUNAN UNIVERSITAS PANCASILA ,
  • KARTININGSIH KARTININGSIH UNIVERSITAS PANCASILA ,
  • LOLA DYAH PITHALOKA UNIVERSITAS PANCASILA ,

Kata Kunci:

brotowali, Tinospora crispa (L.) Miers, flavonoid total, apigenin, α-glukosidase

Abstrak

Dalam upaya pengembangan sediaan  jamu menjadi suatu herbal terstandar perlu dilakukan pemeriksaan terhadap ekstrak yang dapat menjamin khasiat, mutu dan keamanannya. Batang brotowali  (Tinospora crispa (L.) Miers.) diketahui memiliki aktivitas antidiabetes dan tidak toksik. Pada penelitian ini, telah dilakukan uji mutu ekstrak dan uji aktivitas penghambatan enzim α-glukosidase ekstrak etanol kental batang brotowali. Uji mutu ekstrak meliputi penetapan parameter spesifik (identitas, organoleptik, senyawa terlarut dalam pelarut), parameter non spesifik (susut pengeringan, kadar air, kadar abu, sisa pelarut, cemaran  logam berat dan cemaran mikroba) dan penetapan kandungan kimia senyawa marker yaitu penetapan kadar flavonoid total secara spektrofotometri UV-Vis dan penetapan kadar apigenin secara densitometri. Dari hasil pengujian mutu menunjukkan ekstrak memenuhi persyaratan, kadar flavonoid total 0,52% dan kadar apigenin 0,03635%, hasil uji aktivitas penghambatan enzim α-glukosidase diperoleh  IC50 237,26 bpj.

Referensi

Unduhan

Diterbitkan

2014-09-30

Terbitan

Vol 12 No 2 (2014): JIFI

Bagian

Articles

Lisensi

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