Antibacterial activity of an active endophytic Alternaria sp. CATD-09 fraction In vitro and in silico evaluation

Agung Dewantoro; Shirly Kumala; Andria Agusta; Partomuan Simanjuntak

doi:10.35814/jifi.v24i2.2163

Penulis

Agung Dewantoro STIKes Widya Dharma Husada Tangerang, Kota Tangerang Selatan, 15417, Indonesia
Shirly Kumala Doctoral Program Faculty of Pharmacy, Pancasila University, Jakarta, 12640, Indonesia
Andria Agusta Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency, Bogor 16911, West Java, Indonesia
Partomuan Simanjuntak Doctoral Program Faculty of Pharmacy, Pancasila University, Jakarta, 12640, Indonesia

DOI:

https://doi.org/10.35814/jifi.v24i2.2163

Kata Kunci:

Alternaria sp CATD-09, antibacterial, endophytic fungi, in Silico, GC-MS

Abstrak

Endophytic fungi have emerged as promising sources of bioactive secondary metabolites for pharmaceutical discovery due to their ability to produce metabolites similar to those of their host plants. This study investigated the antibacterial potential of strain CATD-09, an endophytic fungus isolated from Cnidoscolus aconitifolius (Mill.) I.M. Johnst., through antibacterial evaluation, chemical characterization, and molecular docking analysis. The antibacterial activity of fungal fractions was assessed against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 6538 using the minimum inhibitory concentration (MIC) method. The most active fraction was subsequently characterized by Gas Chromatography–Mass Spectrometry (GC–MS) to identify its major constituents. To predict the possible antibacterial mechanism, the identified compounds were subjected to molecular docking against penicillin-binding protein 2a (PBP2a), a validated molecular target associated with β-lactam resistance. Fraction 1 exhibited moderate antibacterial activity against S. aureus ATCC 6538 with an MIC value of 128 μg/mL and stronger activity against E. coli ATCC 8739 with an MIC value of 64 μg/mL. GC–MS analysis identified 9,12-octadecadienoic acid (Z,Z)- as the predominant constituent, accounting for 44.47% of the total composition. Molecular docking demonstrated that this compound exhibited a stronger predicted binding affinity toward PBP2a than the reference ligand, indicating its potential to inhibit bacterial cell wall biosynthesis. Overall, these findings suggest that Alternaria sp. CATD-09 is a promising source of antibacterial metabolites and provide a scientific basis for further investigation of its bioactive compounds as potential lead candidates for antibacterial drug discovery.

Referensi

[1] A. Fathoni and M. Ilyas, “Diversity of endophytic fungi from Vernonia amygdalina, their phenolic and flavonoid contents and bioactivities,” Biodiversitas, vol. 21, no. 2, pp. 436–441, 2020, doi: 10.13057/biodiv/d210202.

[2] P. Praptiwi et al., “Bioactivity evaluation of compounds produced by Fusarium equiseti from Kaempferia parviflora rhizome from Indonesia,” J. Appl. Pharm. Sci., vol. 15, no. 7, pp. 179–192, 2025, doi: 10.7324/JAPS.2025.221818.

[3] N. Aji, S. Kumala, E. Mumpuni, and D. Rahmat, “Antibacterial activity and active fraction of Zingiber officinale Roscoe, Zingiber montanum (J. Koenig) Link ex A., and Zingiber zerumbet (L.) Roscoe ex Sm. against Propionibacterium acnes,” Pharmacogn. J., vol. 14, no. 1, pp. 103–111, 2022, doi: 10.5530/pj.2022.14.15.

[4] D. Agung and W. Anjas, “Studi in silico prediksi toksisitas dan aktifitas senyawa xanthoangelol sebagai inhibisi enzim tirosinase,” Edu Masda J., vol. 7, no. 1, 2023, doi: 10.52118/edumasda.v7i1.181.

[5] K. Kusmiati et al., “Profile and in silico analysis of metabolite compounds of the endophytic fungus Alternaria alternata K-10 from Drymoglossum piloselloides as antioxidants and antibacterials,” Heliyon, vol. 10, no. 6, p. e27978, 2024, doi: 10.1016/j.heliyon.2024.e27978.

[6] B. Wagner, Plant Drug Analysis: A Thin Layer Chromatography Atlas, 2nd ed. Berlin, Germany: Springer, 2001.

[7] M. Waksmundzka-Hajnos, J. Sherma, and T. Kowalska, Eds., Thin Layer Chromatography in Phytochemistry. Boca Raton, FL, USA: CRC Press, 2008.

[8] S. Zhao, J. Li, J. Liu, and S. Xiao, “Secondary metabolites of Alternaria: A comprehensive review of chemical diversity and pharmacological properties,” Front. Microbiol., 2023, doi: 10.3389/fmicb.2022.1085666.

[9] X. Shi et al., “New tricycloalternarenes from fungus Alternaria sp.,” J. Asian Nat. Prod. Res., vol. 17, pp. 37–41, 2015, doi: 10.1080/10286020.2014.970536.

[10] Y. Wang et al., “Two new metabolites from the endophytic fungus Alternaria sp. A744 derived from Morinda officinalis,” Molecules, vol. 22, p. 765, 2017, doi: 10.3390/molecules22050765.

[11] M. Wang, Y. Zhang, R. Wang, Z. Wang, et al., “An evolving technology that integrates classical methods with continuous technological developments: Thin-layer chromatography bioautography,” Molecules, vol. 26, p. 4647, 2021, doi: 10.3390/molecules26154647.

[12] D. Sulistiarini, E. Nurul, P. Qodrie, and D. Sahroni, “Antibacterial activity, antioxidant potential, total phenolic and flavonoids of three plant species of Rubiaceae from Banggai Island, Indonesia,” Biodiversitas, vol. 22, no. 5, pp. 2773–2778, 2021, doi: 10.13057/biodiv/d220540.

[13] S. Cai, J. B. King, L. Du, D. R. Powell, and R. H. Cichewicz, “Bioactive sulfur-containing sulochrin dimers and other metabolites from an Alternaria sp. isolate from a Hawaiian soil sample,” J. Nat. Prod., vol. 77, pp. 2280–2287, 2014, doi: 10.1021/np5005449.

[14] A. Dalinova et al., “Isolation and bioactivity of secondary metabolites from solid culture of the fungus Alternaria sonchi,” Biomolecules, vol. 10, p. 81, 2020, doi: 10.3390/biom10010081.

[15] G. Oluwatoyin, S. Samson, A. E. Omolara, and C. Onyebuchi, “Identification of bioactive compounds in the acetone extract of Daedalea elegans using gas chromatography–mass spectrometry: A review,” Int. J. Med. Pharm. Drug Res., vol. 5, no. 5, pp. 1–8, 2021, doi: 10.22161/ijmpd.5.5.1.

[16] K. Gopi, K. Renu, and G. Jayaraman, “Inhibition of Naja naja venom enzymes by the methanolic extract of Leucas aspera and its chemical profile by GC–MS,” Toxicol. Rep., vol. 1, pp. 667–673, 2014, doi: 10.1016/j.toxrep.2014.08.012.

[17] M. N. Abubakar and R. T. Majinda, “GC–MS analysis and preliminary antimicrobial activity of Albizia adianthifolia (Schumach.) and Pterocarpus angolensis (DC),” Medicines, vol. 3, no. 1, 2016, doi: 10.3390/medicines3010003.

[18] B. Chakraborty et al., “Biological activities and GC–MS based chemical profiling of polymolecular methanol extract of Alternaria alternata KUDB15,” J. King Saud Univ. Sci., vol. 35, p. 102884, 2023, doi: 10.1016/j.jksus.2023.102884.

[19] U. S. Onoabedje, C. O. Ezugwu, and E. Abraham, “Antimicrobial properties of 9,12-octadecadienoic acid isolated from leaf extracts of Acalypha fimbriata (Euphorbiaceae),” Commun. Phys. Sci., vol. 12, no. 3, pp. 1061–1069, 2025, doi: 10.4314/cps.v12i3.27.

[20] N. Aji, S. Kumala, E. Mumpuni, and D. Rahmat, “In silico and in vitro antibiofilm activity of Zingiber montanum ethyl acetate fraction against Propionibacterium acnes,” J. Trop. Life Sci., vol. 15, no. 1, pp. 81–92, 2025, doi: 10.11594/jtls.15.01.09.

[21] M. W. Shalaby, E. M. E. Dokla, R. A. T. Serya, and K. A. M. Abouzid, “Penicillin binding protein 2a: An overview and a medicinal chemistry perspective,” Eur. J. Med. Chem., p. 112312, 2020, doi: 10.1016/j.ejmech.2020.112312.

[22] B. Morak-Młodawska and M. Jeleń, “Study of lipophilicity and ADME properties of 1,9-diazaphenothiazines with anticancer action,” Int. J. Mol. Sci., vol. 24, no. 8, p. 6970, 2023, doi: 10.3390/ijms24086970.

[23] Y. Xiao and I. S. Lee, “Effects of microbial transformation on the biological activities of prenylated chalcones from Angelica keiskei,” Foods, vol. 11, no. 4, 2022, doi: 10.3390/foods11040543.

[24] R. Purushothaman, G. Vishnuram, and T. Ramanathan, “Isolation and identification of n-hexadecanoic acid from Excoecaria agallocha L. and its antibacterial and antioxidant activity,” preprint, 2024, doi: 10.2139/ssrn.4886224.