Aktivitas Antibakteri Isolat bakteri X2 yang Berasosiasi Spons Xestospongia testudinaria dari Pantai Pasir Putih Situbondo terhadap Bakteri Pseudomonas aeruginosa

Penulis

  • YATNITA PARAMA CITA UNIVERSITAS INDONESIA ,
  • OCKY KARNA RADJASA UNIVERSITAS DIPONEGORO ,
  • PRATIWI SUDHARMONO UNIVERSITAS INDONESIA ,

Kata Kunci:

isolat X2-10, P. aeruginosae, antimikroba, B. licheniformis

Abstrak

This study was conducted to isolate and test the antibacterial activity of bacterial isolate X2 associated with Xestospongia testudinaria sponge from Pasir Putih Beach Situbondo. Bacterial isolation was carried out by pour plate method then bacteria colony obtained was purified by streak plate method. Isolate bacteria X2 produced 12 bacterial isolates which was tested for antibacterial activity against test bacterias of Klebsiella pneumonia, Pseudomonas aeruginosa and Salmonella typhii using overlay method. The results demonstrated that obtained isolate X2-10 was active against P. aeruginosa ATCC27853 and MDR using agar diffusion method. Characterization of isolate X2-10 was done by Gram staining test, biochemical test and identification with 16s-rRNA. The results showed that bacterial isolates X2-10 associated with X. testudinaria sponge displayed an antimicrobial activity against P. aeruginosa ATCC27853 and MDR. Based on the characterization result, the bacterial isolate X2-10 was B. licheniformis.

Referensi

Unduhan

Diterbitkan

2017-09-04

Terbitan

Vol 14 No 2 (2016): JIFI

Bagian

Articles

Lisensi

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