Aktivitas Antimalaria Ekstrak n-Hexana Daun Artemisia Cina Galur Iradiasi terhadap Plasmodium berghei ANKA

Abstrak

Mutation techniques with irradiation has been shown to increase the artemisinin content of the leaves. In this study antimalarial activity test of leaf extracts radiation strains 10, 20, and 40 Gy and dose optimization of therapy aims to get leaf extract which has antimalarial activity of the best and optimum therapeutic dose. Research phase radiation dose optimization is done in vivo using mice infected with 1 x 107 P. berghei were divided into 4 groups: positive control, radiated 10 Gy, 20 Gy, 40 Gy. Giving artemisinin conducted for 3 days after the degree of parasitaemia has reached 5%. Observations parasitaemia was performed daily until day 7 post-treatment. The survey results revealed artemisinin 10 Gy provide the best results for the lowest parasite growth and the mice live longer. Phase therapeutic dose optimization performed by the same method with a variety of therapeutic doses of 100, 200, and 300 mg / kg. The results showed the mutant strains leaf extract can inhibit the growth P.berghei significantly (p <0.05) against a control with ED 238,7 mg/kg body weight. Conclusion leaf extract mutant 10 Gy with efectif of therapeutic dose (ED50) 238,7 mg / kg BW.