Isolasi Sel Punca Pluripoten dengan Penanda CD105+ dan SSEA3+ dari Sel Fibroblas Kulit asal Jaringan Preputium

Penulis

  • CHURIYAH CHURIYAH Badan Pengkajian dan Penerapan Teknologi, Kawasan Puspitek ,
  • INDRA KUSUMA UNIVERSITAS YARSI ,
  • SISKA A KUSUMASTUTI Badan Pengkajian dan Penerapan Teknologi, Kawasan Puspitek ,
  • RESTU SYAMSUL HADI UNIVERSITAS YARSI ,
  • AGUNG ERU WIBOWO Badan Pengkajian dan Penerapan Teknologi, Kawasan Puspitek ,
  • FAIZA KARA FABIOLA Badan Pengkajian dan Penerapan Teknologi, Kawasan Puspitek ,

Kata Kunci:

fibroblasts, selpunca CD105 ,, sel punca SSEA3 , alkalin fosfatase

Abstrak

The development and knowledge of stem cell research are growing quickly. Stem cells are now likely to become a modern treatment as a regenerative approach that has potential treatment for various diseases that are difficult to cure, such as genetic diseases, degenerative, trauma, and malignancy. The aimed of this study was to isolate pluripotent stem cells using human dermal fibroblast cell obtained from preputium tissue by enzymatic and tissue explants methods, followed by double positive characterization for specific markers of cluster differentiation (CD) 105 and surface-stage embryonic antigen (SSEA)-3 using magnetic-based purification system (MACS). The fibroblast cells resulted from both method were cultured and expanded by variations medium and passage cells, then were characterized for double positive CD 105+ and SSEA3+. Fibroblast cell culturing in conditioned medium, lower passage and higher density has the highest percentage of CD105+ subpopulation cells compared than fibroblast cell in standard medium, higher passage and lower density. Subpopulation of both of CD 105+ and SSEA3+ cells revealed a positive response to alkaline phosphatase dye which proving a population of stem cells.

Referensi

Unduhan

Diterbitkan

2017-09-04

Terbitan

Vol 14 No 2 (2016): JIFI

Bagian

Articles

Lisensi

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