Aktivitas Antibakteri Ekstrak Etil Asetat Media Kultur Fungi Endofi t Nigrospora oryzae dari Rhizophora mucronata
DOI:
https://doi.org/10.35814/jifi.v15i2.516Kata Kunci:
Aktivitas antibakteri, Nigrospora oryzae, Rhizophora mucronataAbstrak
Tujuan penelitian ini adalah untuk memberikan informasi mengenai sifat antibakteri ekstraketil asetat media kultur fungi endofi t daun bakau (R. mucronata).Fungi endofit yang telah diisolasi dari daun R. mucronata diidentifikasi secara makroskopik, mikroskopik, dan molekuler. Ekstrak etil asetat dari media kultur fungi endofi t diuji aktivitas antibakterinya pada Staphylococcus aureus dan Escherichia coli menggunakan metode mikrodilusiuntuk mendapatkan nilai Konsentrasi Hambat Minimum (KHM). Hasil penelitian diperoleh isolat fungi endofi t yaitu Nigrospora oryzae. Ekstrak etil asetat (EtOAc) media kultur fungi endofi t yang diujikan pada E. coli menunjukkan nilai KHM 250 μg/mL terhadap bakteri E. coli dan 500 μg/mL terhadap bakteri S. aureus.
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