Daya Antibakteri Ekstrak dan Fraksi-Fraksi Daun Jambu Mete (Anacardium occidentale L.) terhadap Bakteri Staphylococcus aureus Sensitif dan Multiresisten

Penulis

  • YULIANA RIZQI DWI RATNA UNIVERSITAS MUHAMMADIYAH SURAKARTA ,
  • UTARI SITA ARDANI UNIVERSITAS MUHAMMADIYAH SURAKARTA ,
  • ZAKIAH FATHIANA UNIVERSITAS MUHAMMADIYAH SURAKARTA ,
  • ANNIE RAHMATILLAH UNIVERSITAS MUHAMMADIYAH SURAKARTA ,
  • IKA TRISHARYANTI D K UNIVERSITAS MUHAMMADIYAH SURAKARTA ,

Kata Kunci:

Anacardium occidentale L, Staphylococcus aureus, antibakteri, kromatografi lapis tipis

Abstrak

Cashew leaves have antibacterial activity against Gram positive and negative bacteria that are sensitive and multiresisten. The aim of this study is to determine the antibacterial activity of extracts and fractions of cashew leaf and determine the content of its compounds. The extraction method was performed by maceration using 96% ethanol. Fractionation of ethanol extract of cashew leaves was done partitioning method. Extract and fractions were tested for antibacterial activity using solid dilution method to observed levels of minimum inhibitory concentration (MIC). The results of the MIC continued to minimum bactericidal concentration (MBC). Analysis of the active constituent was carried by thin layer chromatography (TLC). The results showed that tehanol extract, chloroform fraction and ethyl acetate fraction has the power to kill the sensitive and multiresistant.Staphylococcus, whereas for methanol-water fraction up to 2% levels showed no killing power against sensitive S. aureus and levels of 3% against the multiresistant Staphylococcus. The result of TLC test showed that the extract and fractions of cashew leaf contains alkaloids, flavonoids, essential oils, and phenols.

Referensi

Unduhan

Diterbitkan

2017-09-04

Terbitan

Vol 14 No 1 (2016): JIFI

Bagian

Articles

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