Formulasi dan Uji Inhibitor Tirosinase Masker Peel-Off Ekstrak Etanol 96% Kulit Buah Jeruk Nipis (Citrus aurantifolia)
DOI:
https://doi.org/10.35814/jifi.v19i1.764Kata Kunci:
Kulit jeruk nipis, masker peel-off, inhibitor tirosinaseAbstrak
Tirosinase merupakan enzim yang secara luas terdistribusi dalam mikroorganisme, tumbuhan dan
binatang. Tirosinase adalah enzim utama yang terlibat dalam biosintesis melanin. Melanin mempunyai peran yang sangat penting dalam perlindungan kulit terutama terhadap paparan sinar ultraviolet yang berbahaya bagi kulit. Penelitian ini bertujuan untuk menguji inhibitor tirosinase pada masker peel-off ekstrak kulit jeruk nipis (Citrus aurantifolia). Ekstrak kulit jeruk nipis diformulasikan menjadi masker peel-off dengan kandungan ekstrak masing-masing 0% (F1), 15% (F2) dan 25% (F3) dan dengan gelling agent PVA ( Polivinil alkohol). Uji kestabilan fi sik dilakukan dengan pengukuran pH diperoleh hasil F2 pH 6,85 dan F3 pH 6,78. Analisis aktivitas inhibitor tirosinase dilakukan menggunakan spektrofotometer UV-Vis dan dilihat dari nilai persen inhibisi yang didapatkan. Hasil persen inhibisi yang diperoleh dari masker peel-off ekstrak jeruk nipis yaitu 17,89 % (F2) dan 18,86 % (F3). Kulit jeruk nipis berpotensi sebagai inhibisi tirosinase.
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