Antioxidant Activity of Combination between Ginger Extract (Zingiber officinale Rosc.) with Zink Based on MDA, SOD and Catalase Measurements in Hypercholesterolemia and Hyperglycemia Mice with Streptozotocin as Inducer

Abstrak

Hypercholesterolemia and hyperglycemia were able to trigger the free radicals excess. To prevent this case, antioxidant administration is needed. The combination of ginger extract (Zingiber officinalle Rosc.), a plant that contains antioxidants, with zink is expected to improve the ability of antioxidants to fight free radicals that cause damage to the organs. The purpose of this study was to determine antioxidant activity of combination between ginger extract and zink by measuring the levels of malonyldialdehide (MDA), superoxyde dismutase (SOD) and catalase in hypercholesterolemia and hyperglycemia mice with streptozotocin as inducer. 48 male DDY mice were divided into 8 groups, with 6 mice in each group. Group I (normal), group II (negative), group III (atorvastatin), group IV (ginger extract 37.5 mg /kgbw), group V (zink 20mg/kgbw), group VI (ginger extract 37.5 mg/kgbw and zink 20mg/kgbw), group VII (75mg/kgbw ginger extract and zink 20mg/kgbw) and group VIII (150mg/kgbw ginger extract and zink 20mg/kgbw). Measurement of MDA, SOD and catalase in mice’s livers and bloods was conducted using a UV-Vis spectrophotometer. The data obtained were tested by one -way ANOVA, followed by Tukey test. The results showed that group VII was able to reduce levels of MDA and increase levels of SOD and catalase in mice much better than others. This antioxidant activity is comparable to that of the positive control.