Antifungal Activity of Streptomyces Isolate Associated with The Rhizosphere of Belulang Grass (Eleusine indica)

Abstrak

The appearing of various new infection diseases and resistantly pathogenic microorganism to old antibiotic, have motivated another antibiotic discovery from bacteria, fungus and Actinomycetes. Nowadays, some researchers are being focused on Actinomycetes, that is indicated as the bacteria which produces the biggest number of antibiotics. About 45,6% of 8.000 antimicrobia were produced by Streptomyces. The aims of this research were to isolate and identify of Streptomyces from rhizosphere of belulang grass (Eleusine indica) that potentially as antibiotic producer. This research was exploration research. From this research, 33 isolates from rhizosphere of belulang grass (Eleusine indica) were found, 17 isolates potentially as antifungal producer. Eight isolates could inhibit only one of test fungal, seven isolates could impede the growth of two test fungal and one isolate (RRR67) could inhibit the growth of three test fungal, namely Trichophyton mentagrophytes, Aspergilus fumigatus and Candida albicans. Based on SEM result, it could be known that the spore morphology and the ornament of the spore chain surface, one isolate (RRR67) was identified as Streptomyces. From the research, it could be concluded that 17 isolates from the rhizosphere of belulang grass (Eleusine indica) potentially produce antifungal which is need for infection medicine treatment.